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Flow cytometry is a technique for analysing single cells or particles as they pass through one or more lasers.

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Shivani
Flow cytometry is a technique for analysing single cells or particles as they pass through one or more lasers.

Flow cytometry is a technique for analysing single cells in solution quickly and with multiple parameters. Flow cytometers use lasers as light sources to generate scattered and fluorescent light signals, which are detected by photodiodes or photomultiplier tubes. These signals are transformed to electrical signals, which are then processed by a computer and written to a standardised format data file (.fcs). Based on their fluorescent or light-scattering features, cell populations can be studied and/or isolated.


Flow cytometry makes use of a range of fluorescent reagents. Fluorescently conjugated antibodies, DNA binding dyes, viability dyes, ion indicator dyes, and fluorescent expression proteins are examples of these.


Flow cytometry is a versatile technology used in immunology, molecular biology, bacteriology, virology, cancer biology, and infectious disease surveillance. Over the last 30 years, it has advanced dramatically, allowing for unparalleled detail in studies of the immune system and other aspects of cell biology.


Read more @ https://tradove.com/blog/Flow-cytometry-is-a-popular-technique-for-determining-the-expression-of-cell-surface-and-intracellular-chemicals.html



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